aeruginosa in patients and hospital surfaces. Our results showed high abundances of HAI-related bacteria such as S. In this paper, we used these methods to perform a one-year cross sectional profiling of bacteria and AMR genes in adult and neonatal intensive care units (ICU and NICU) in a Brazilian public, tertiary hospital.
Bacterial high-throughput amplicon gene sequencing along with identification of AMR genes, as well as whole genome sequencing (WGS), are biotechnological tools that allow multiple-sample screening for a diversity of bacteria. These HAI-related bacteria contribute to a major public health issue by increasing patient morbidity and mortality during or after hospital stay.
Frequently, these bacteria are related to HAI (healthcare-associated infections) and carry antimicrobial resistance (AMR). Several studies have shown the ubiquitous presence of bacteria in hospital surfaces, staff, and patients. Ana Paula Christoff, Data curation, Formal analysis, Methodology, Writing – original draft, Writing – review & editing, 1 Aline Fernanda Rodrigues Sereia, Conceptualization, Data curation, Formal analysis, Methodology, Project administration, Supervision, Writing – review & editing, 1 Giuliano Netto Flores Cruz, Data curation, Formal analysis, Validation, Writing – review & editing, 1 Daniela Carolina de Bastiani, Formal analysis, Investigation, 1 Vanessa Leitner Silva, Data curation, Formal analysis, Investigation, 1 Camila Hernandes, Conceptualization, Project administration, Supervision, 2 Ana Paula Metran Nascente, Investigation, 2 Ana Andrea dos Reis, Investigation, 2 Renata Gonçalves Viessi, Investigation, 2 Andrea dos Santos Pereira Marques, Investigation, 2 Bianca Silva Braga, Investigation, 2 Telma Priscila Lovizio Raduan, Conceptualization, Investigation, Methodology, Project administration, Supervision, 2 Marines Dalla Valle Martino, Conceptualization, Methodology, Project administration, 2 Fernando Gatti de Menezes, Conceptualization, Project administration, 2 and Luiz Felipe Valter de Oliveira, Conceptualization, Data curation, Formal analysis, Methodology, Project administration, Supervision, Writing – review & editing 1, *
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